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Paper alert! Now published @stemcellreports:
Our analysis of the recruitment of the Polycomb PRC2 complex to DNA in mouse embryonic stem cells
1/ https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(20)30284-8
Our analysis of the recruitment of the Polycomb PRC2 complex to DNA in mouse embryonic stem cells
1/ https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(20)30284-8
The paper consititutes a resource of 868 million mapped sequencing reads of high quality ChIP profiles of ES cells, in addition to whole cell and chromatin-associated proteomes.
Easy access through our ChIP track data hub:
2/
http://veenstra.science.ru.nl/trackhubm.htm
Easy access through our ChIP track data hub:
2/
http://veenstra.science.ru.nl/trackhubm.htm
We used null mutations PRC2 and PRC1 in combination with EED inhibition, to quantify contributions to Polycomb binding across the genome
Some of our findings:
3/
Some of our findings:
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PRC2.1 binding to DNA by MTF2, PRC2.2 recruitment through JARID2-H2Aub-PRC1, both stabilized by EED interaction with H3K27me3
The JARID2 and EED-mediated interactions are partially redundant
PRC1, PRC2.1, and PRC2.2 are all mutually interdependent for binding to chromatin
4/
The JARID2 and EED-mediated interactions are partially redundant
PRC1, PRC2.1, and PRC2.2 are all mutually interdependent for binding to chromatin
4/
Our work is related to papers published last November (Healy et al, Højfeldt et al: https://tinyurl.com/ybnko774 )
History:
06-2019 posted on @bioRxiv https://tinyurl.com/y8t6abwx
07-2020 accepted @stemcellreports
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History:
06-2019 posted on @bioRxiv https://tinyurl.com/y8t6abwx
07-2020 accepted @stemcellreports
5/
Unique to our paper is chemical inhibition of EED in combination with Mtf2, Jarid2 and Ring1a/b null mutations
This uncovers some unexpected functional interactions
For example, the loss of JARID2 results in a much more dramatic loss of PRC2 binding when EED is also inhibited
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This uncovers some unexpected functional interactions
For example, the loss of JARID2 results in a much more dramatic loss of PRC2 binding when EED is also inhibited
6/
Good to see the work now published
It's open access. I hope it will be read and that people will make good use of the data
Great work by @MatteoPerino_ @GuidoMierlo and other colleagues in collaboration with @hendrik_marks @Radboud_Uni
7/7
It's open access. I hope it will be read and that people will make good use of the data
Great work by @MatteoPerino_ @GuidoMierlo and other colleagues in collaboration with @hendrik_marks @Radboud_Uni
7/7
